Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing

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Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing

BACKGROUND Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive resul...

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Coping with cross-reactive carbohydrate determinants in allergy diagnosis

A relevant proportion of allergy diagnosis is accomplished by in vitro determination of specific immunglobulin E (sIgE) to extracts from suspected allergens. Such extracts inevitably contain glycoproteins, which may react with patients' IgE. In the case of plant and insect allergens, the relevant epitope structure is an α-1,3-fucose on the Asn-linked sugar residue of so-called N-glycans. Due to...

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Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity

BACKGROUND Allergy diagnosis by determination of allergen-specific IgE is complicated by clinically irrelevant IgE, of which the most prominent example is IgE against cross-reactive carbohydrate determinants (CCDs) that occur on allergens from plants and insects. Therefore, CCDs cause numerous false-positive results. Inhibition of CCDs has been proposed as a remedy, but has not yet found its wa...

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Allergy testing in childhood: using allergen-specific IgE tests.

A variety of triggers can induce common pediatric allergic diseases which include asthma, allergic rhinitis, atopic dermatitis, food allergy, and anaphylaxis. Allergy testing serves to confirm an allergic trigger suspected on the basis of history. Tests for allergen-specific immunoglobulin E (IgE) are performed by in vitro assays or skin tests. The tests are excellent for identifying a sensitiz...

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Allergy Testing in Childhood: Using Allergen-Specific IgE Tests abstract

A variety of triggers can induce common pediatric allergic diseases which include asthma, allergic rhinitis, atopic dermatitis, food allergy, and anaphylaxis. Allergy testing serves to confirm an allergic trigger suspected on the basis of history. Tests for allergen-specific immunoglobulin E (IgE) are performed by in vitro assays or skin tests. The tests are excellent for identifying a sensitiz...

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ژورنال

عنوان ژورنال: Asia Pacific Allergy

سال: 2017

ISSN: 2233-8276,2233-8268

DOI: 10.5415/apallergy.2017.7.1.29